Let’s try,
measurement
of exosomes
and extracellular vehicles!
Introduction
Exosomes/extracellular vesicles (EVs) are nano vesicles that are secreted from cells and act on other cells. They contain a variety of factors inside and on their surface, which can be quantified by using them as markers.
However, for many species, markers have not been identified, making the measurement of exosome EVs difficult.
GIFU EXOSOME Co. LTD. can measure exosomes and EVs even for species with unidentified markers.
Service
GIF-2250, fluorescent ｃompounds for exosome and EV staining.
In addition, GIF-2250 can stain late endosomes and lysosomes as a live imager.
For 100 assays 〜
Contract measurement of exosomes and EVs
(Rapid purification & Quantification)
Establish the methods for exosomes and EVs
(Optimization of the purification and storage)
From 1 case 〜
Others: electron microscopy, size measurement, labeling
Development of products containing exosomes and EVs
Identification of physiological effects of exosomes and EVs
Exosomes and EVs can actually be found in large quantities in commercially available foods.
Example) Fermented foods and vegetables
This is a joint research with Gifu University.
Business model
I want to develop my own new product related to extracellular vesicles (EV)
First, check the EV with GIFU EXOSOME.
I want to incorporate GIFU EXOSOME technology
We support everything from simple EV purification to large-scale purification, depending on the material.
Supports direct measurements from cultured cell culture media.
I want to try a product developed by GIFU EXOSOME
Mainly derived from fermented foods.
GIFU EXOSOME
Solutions
Patents (Gifu University)
GIF-2250
JP 2021-012443 PCT/JP2022/1692 （USA, UK, Fra, Germa, China）
「Compounds, cellular vesicle staining agents, and methods of fluorescent staining of cellular vesicles」
Gifu Univ. 2021/01/28
GIF-2276
JP 2022-141909
「Compounds, extracellular vesicle staining agents, and fluorescent staining methods for extracellular vesicles」
Gifu Univ. 2022/09/07
(GIFU EXOSOME Co, Ltd. )
A. lavenia
JP Patent No. 7262319
「Melanin generation inhibitor and method for manufacturing melanin generation inhibitor」
GIFU EXOSOME Co. Ltd. 2019/01/14
Publications (Gifu University)
GIF-2250
Intermittent inhibition of FYVE finger-containing phosphoinositide kinase induces melanosome degradation in B16F10 melanoma cells. Mol Bio Rep in press
https://doi.org/10.1007/s11033-023-08536-9Visualization of mitophagy using LysoKK, a 7-nitro-2,1,3-benzoxadiazole-(arylpropyl)benzylamine derivative Mitochondrion 2022 62: 176-180
https://doi.org/10.1016/j.mito.2021.12.004Identification of novel neuroprotective N,N-dimethylaniline derivatives that prevent oxytosis/ferroptosis and localize to late endosomes and lysosomes Free Radical Biology and Medicine 2021 174: 225-235
https://doi.org/10.1016/j.freeradbiomed.2021.08.015GIF-2276
under construction
References
Methods for Quantifying Extracellular Vesicles (EVs) Across Species Monthly Bioindustry 2022 Dec.
A. lavenia
Diversity of Adenostemma lavenia, multi-potential herbs, and its kaurenoic acid composition between Japan and Taiwan Journal of Natural Medicines 2022 76: 132-143
https://doi.org/10.1007/s11418-021-01565-3The High Content of Ent-11α-hydroxy-15-oxo-kaur- 16-en-19-oic Acid in Adenostemma lavenia (L.) O. Kuntze Leaf Extract: With Preliminary in Vivo Assays. Foods (Basel, Switzerland) 2021 9: 1
https://doi.org/10.3390/foods9010073About Us
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